Our services include:

-Sample trimming (grossing)

-Paraffin wax processing (FFPE)

-Frozen/Cryo

-Embedding (Formalin Fixed Paraffin Embedded - FFPE / OCT embedded - Cryo)

-Sectioning

-Staining

Specialized Histology

-Ocular Histology

-Special Stains

-Necropsy Support including prosecutor service and training

Specialized Stains

We offer a large selection of special stains, below are some of our most popular options. We provide tailor made experimental design to suit your needs.

-Alcian Blue (Proteoglycan)

-Alizarin Red (Calcium Salts)

-Congo Red (Amyloid)

-Cresyl Violet (Neurons)

-Giemsa (Bone Marrow, Helicobacter pylori, and mast cells)

-Jones’s Silver (Basement Membranes)

-Luxol Fast Blue (Mylelin and Nissil substance)

-Masson Trichrome (Collagen)

-Mucicarmine (Mucins)

-Oil Red O (Fat and Lipid)

-Periodic Acid-Schiff (Glycogen)

-P.T.A.H (Fibrin, Muscle)

-Safrarnin O (Cartiliage)

-Sudan Black (Fat and Lipid)

-Van Gieson’s (Collagen)

Immunohistochemical or Immunofluorescent staining is widely used as a diagnostic tool to visualize abnormal cells such as those found in cancerous tumors. Specific antibodies will bind their target antigens in paraffin or cryo tissue sections. This technique enables the researcher to gain a better understanding of a particular protein distribution and localization of biomarkers and differentially expressed proteins in different part of the biological tissue. This technique can be done using either traditional brightfield microscopy which utilized 3,3-Diaminobenzidine (DAB - brown) to visualize the immuno products and Mayers’ hematoxylin is commonly used as a nuclear counterstain or immunofluorescent microscopy where a fluorescent dye is used and 4',6-diamidino-2-phenylindole (DAPI) as a counterstain.

At SDHistoPath, we offers a large selection of established protocols, below are just some of our most popular IHC markers. We also provide tailor made multiplexing experimental design to suit your study needs.

Please contact us using the form below to speak with a scientist or obtain a quote for our immunohistochemistry services.

-CD4

-CD8

-CD31

-CD68

-B220

-Ki67

-PCNA

-Caspase 3

-Human Mitochondria

-VEGF

-C-Myc

-GFAP

-Myosine

-Pax 7

-Β Tubulin

-Cadherin

-p53

-Phospho Histone H3

-Akt

-MEK1/2

-SOX2

-BrdU

-GFP

-RFP

-tdTomato

In Situ hybridization is a technique to detect the expression pattern of a particular gene sequence in cells or tissues at the nucleic acid level using a complimentary sequence known as a probe. The probes are typically labeled with a fluorochrome or an enzyme so they can be detected either by using a fluorescent microscope or a color substrate which is visible by brightfield microscope.

The colorimetric or fluorescent In Situ hybridization we use here at SDHistoPath is a quantitative, highly sensitive technique that can detect one single gene in a cell by highlighting it as a colored dot. These dots can then be counted by the researcher or by image analysis software to get numerical data.

Please contact us using the form below to discuss your project or obtain a quote for our in situ hybridization services.

We offer the following ISH services:

-DNAscope

-RNAscope

-RNA Multiplex

-TUNEL Assay

At SDHistoPath, we offer brightfield and fluorescent whole slide imaging. The resulting images are high resolution, publication ready, and compatible with a wide variety of image analysis and editing programs.

Additionally, we offer image analysis for standardized quantification of various aspects of your study. Please contact us using the form below to go over your specific image analysis request or to obtain a quote.

Study scoring is a crucial step for analyzing and translating your data into results. At SDHistoPath our highly qualified veterinary pathologist has over 25 years of experience in toxicologic pathology and toxicology (GLP and Investigative/Discovery) across numerous areas including small molecules, proteins, vaccines, chemicals (EPA) and regulatory submissions (FDA, EMEA, drugs and devices).

Our pathologist is triple Board Certified in Toxicology (ABT), Veterinary Pathology (ACVP) and Laboratory Animal Medicine (ACLAM)

Necropsy is the foundation of any successful histologic study. Improper tissue collection will lead to inconsistent or incomplete data regardless of the steps and measures taken downstream. Every step in the histology workflow (necropsy, fixation, embedding, sectioning, antigen retrieval, etc.) has unique challenges that can derail your experiments and compromise your results.

SDHistoPath has over 30 years of experience with every facet of histologic studies in pharmaceutical, clinical and research settings. We can provide tailor made consultation to suit your specific needs with both murine and non-murine samples. Below is a list of some of the areas we can help with:

-Prosector Training

-Specialized tissue harvest

-Blood collection

-Cerebral spinal fluid (CSF) collection

-Small animal perfusion

-Tissue trimming

-Specialized tissue fixation

-Study Planning

-Antibody Selection for multiplex staining